Cross-species transferability of microsatellite markers in the genus Lippia.
نویسندگان
چکیده
The cross-species transferability of 20 microsatellite markers was tested in the genus Lippia. Eleven markers were polymorphic after screening 19 accessions of Lippia sidoides and Lippia gracilis maintained in the Active Germplasm Bank (AGB) from Universidade Federal de Sergipe. Additionally, 40 accessions of Lippia spp were collected in Sergipe to increase the germplasm bank. A total of 23, 22, and 36 alleles were identified, with an average of 2.3, 2.2, and 3.27 alleles per locus, respectively, for each group. The markers that were used were efficient tools to access genetic diversity in the germplasm bank and will be useful for further research aiming at the conservation and management of these important aromatic species.
منابع مشابه
Transferability of barley microsatellite markers for assessing genetic relationships of wheat wild relatives of Triticum and Aegilops genera
Comparative genomics has revealed a high degree of collinearity between related species, which allows transferability of interspecies and intergenus markers. The aim of this study was to examine the transferability of barley microsatellite markers among species of Triticum and Aegilops genus for determination of their genetic relationships. The experiments was carried out in faculty of agricu...
متن کاملApplication of Random Amplified Microsatellite Polymorphism (RAMP) in Prunus Characterization and Mapping
Random amplified microsatellite polymorphism (RAMP) is a PCR-based marker which uses a combination of two classes of markers: Simple sequence repeat (SSR) and Random amplified DNA polymorphism (RAPD) markers. RAMP has been demonstrated to be a potentially valuable molecular marker for the study of genetic relationships in cultivated plant species. The objective of this study was to optimize the...
متن کاملDevelopment of a novel set of microsatellite markers for Lippia alba (Verbenaceae).
Microsatellite primers were developed and optimized for Lippia alba to characterize the L. alba germplasm bank of Universidade de São Paulo. A genomic library enabled the design of 9 microsatellite primers. Six of the 9 primers yielded polymorphic products, which defined 2 groups in the bank. The data provide support to characterize germplasm banks, genetic breeding programs for L. alba, and ot...
متن کاملDevelopment of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae)1
UNLABELLED PREMISE OF THE STUDY This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • METHODS AND RESULTS Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 ma...
متن کاملPhylogenetic and microsatellite markers for Tulasnella (Tulasnellaceae) mycorrhizal fungi associated with Australian orchids1
UNLABELLED PREMISE OF THE STUDY Phylogenetic and microsatellite markers were developed for Tulasnella mycorrhizal fungi to investigate fungal species identity and diversity. These markers will be useful in future studies investigating the phylogenetic relationship of the fungal symbionts, specificity of orchid-mycorrhizal associations, and the role of mycorrhizae in orchid speciation within ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Genetics and molecular research : GMR
دوره 13 4 شماره
صفحات -
تاریخ انتشار 2014